Assessment of Factor VII and Factor X

Summary

A method is described to separate factor VII from factor X in normal serum using electrophoresis in a polyvinyl chloride block. A potent source of factor X is obtained. This may be used in correction studies and for assessment of deficiencies of factor VII in patients.

• References

• 1 Adamis D, Sise M. S, Kimball D. M. The proconvertin test. A simplified method and its application to the study of anticoagulant processes. J. Lab. clin. Med 47: 320 1956;
• 2 Böttiger L. E, Norberg R. Zone electrophoresis in polyvinyl chloride. Clin. chim. Acta 09: 82 1964;
• 3 Denson K. W. The specific assay of Prower-Stuart Factor and Factor VII. Acta, haemat. (Basel) 25: 105 1961;
• 4 Ditterbrandt M. Application of the Weichselbaum Biuret reagent to the determination of spinal fluid protein. Amer. J. clin. Path 18: 439 1948;
• 5 Duckert F, Koller F, Matter M. Purification and physiological properties of Factor VII from plasma and serum. Separation from prothrombin. Proc. Soc. exp. Biol. (N.Y) 82: 259 1953;
• 6 Duckert F, Yin E. T, Straub W. Separation and purification of the blood clotting factors by means of chromatography and electrophoresis. Protides of the Biological Fluids 1960. Peeters H. (ed.). 41 Elsevier; Amsterdam: 1961
• 7 Fisch U. Über einen neuen Accelerator der Blutthrombokinasebildung. Thrombos. Diathes. haemorrh. (Stuttg) 02: 60 1958;
• 8 Gedin H. I, Porath J. Studies of zone electrophoresis in vertical columns. II. Zone electrophoresis of serum protein. Biochim. biophys. Acta 26: 159 1957;
• 9 Prydz H. Studies on proconvertin, I. Gelnitration. Scand. J. clin. Lab. Invest 15: 450 1963;
• 10 Prydz H. Studies on proconvertin (Factor VII), II. Purification. Scand. J. clin. Lab. Invest 01: 101 1964;
• 11 Shaw S, Pegrum G. D, Wolff S. Variations in coagulation factors in patients on anticoagulant therapy. J. Path. Bact 87: 462 1964;
• 12 Straub W. Separation of coagulation factors by means of starch gel electrophoresis. Thrombos. Diathes. haemorrh. (Stuttg) 04: 451 1960;